Interactive effect of light quality and temperature on Chlamydomonas reinhardtii growth kinetics and lipid synthesis

The efficient operation of an algal photobioreactor (PBR) requires appropriate lighting conditions. Despite recent advantages in PBR design and operation, optimal conditions are still not fully understood. This work focuses on the effect light quality culturing temperature growth kinetics lipid content. green alga Chlamydomonas reinhardtii was grown batch culture a constant-temperature incubator-shaker under LED light. Three wavelength regimes were studied: blue (peaking at 433 nm–447 nm, 458 nm–470 nm), white-yellow 456–458 nm blue, 545 570 yellow) red-orange 580 nm–594 604 yellow-orange, 630 656 red, 735 IR). Results show that obtained 38% higher biomass productivity than 24 °C, 13% 32 °C. Red-orange or temperatures 30–32 °C favored accumulation with mass fraction concentration 44% 80% higher, respectively, compared to In late exponential phase, when nutrients medium sufficient, predominant factor controlling synthesis accumulation. While stationary phase nutrient starvation, strong interactive detected between (P = 0.02) for Under both stress, there reduced synthesis.